In the movie Resident Evil: Apocalypse, Jill is portrayed by Sienna Guillory. Early in the film, a newspaper article in her apartment hints at her dismissal from the S.T.A.R.S. unit and her involvement with Nemesis. Both add to similarities between the movie and the game Resident Evil 3: Nemesis. Although, due to the vastly different conclusion, as well as the role of Alice, the game and movie are officially considered alternate universes.
In Resident Evil: Apocalypse, Jill had been a rising star in the S.T.A.R.S. organization, before her reputation was ruined after a botched mission known as "The Arklay Mountains Incident". Information from the movie's website indicates that she gained insight into a "classified bioweapon weakness", which was said to be a "cranial flaw". Initially the Umbrella Corporation intended to bribe her, so their bioweapons wouldn't lose market value. Had that failed, they planned to have her "neutralized". In the film, Jill maintains a strong friendship with S.T.A.R.S. officer Peyton Wells. During a T-virus outbreak in Raccoon City, Jill attempted to leave the city through a quarantine screening set up by Umbrella at Ravens Gate Bridge. When the infection reached the Gates, Umbrella sealed off the sector and urged that all citizens return to their homes.
Upon heading back to the city, Jill stumbled upon news reporter Terri Morales, who managed to video tape Umbrella's actions at the Bridge. When Jill decides to hold up at Ravens Gate Church, her group is attacked by Lickers. Alice manages to locate Jill and her companions whom are hiding out. It is notable that Jill is far less self-sufficient in the movie, and likely would have died if not for the help of Alice. When Umbrella intervenes Dr. Ashford's plan to use Alice to get his daughter Angela out of the city in exchange for a safe departure out of the city. Jill is taken hostage by Umbrella soldiers and is later released by Carlos during a gunfight (with Umbrella agents) against Alice at Raccoon City Hall. Before the destruction of Raccoon City, Jill manages to get the survivors out. Upon departure the helicopter is hit by the blast wave and crashes into the Arklay Mountains. All except Alice survive the crash; the remaining survivors flee the scene.